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agei restriction cut sites  (New England Biolabs)


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    Structured Review

    New England Biolabs agei restriction cut sites
    Agei Restriction Cut Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agei+restriction+cut+sites/AgeI/10__1091_slash_mbc__e23___06___0257-250-20-46
    Average 97 stars, based on 870 article reviews
    agei restriction cut sites - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes
    Article Snippet: SS (lactase)-S-FLAG-AAT or SS (lactase)-S-FLAG-ATZ: “S-FLAG” refers to the SNAP-FLAG, which was derived from SNAP-FLAGCD59 (obtained from Addgene, plasmid # 50374, deposited by Reika Watanabe) by PCR amplification.

    Generated:

    Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes
    Article Snippet: For assembly we used NEBuilder (NEB E5520S). .. SS(lactase)-VEN-AAT and SS(lactase)-VEN-ATZ: These were generated by starting with SS(lactase)-CER-TMED9 (described above) and replacing CER with VEN through BsrGI and AgeI restriction cut sites, and then replacing the TMED9 mature domain with AAT and ATZ mature domains cloned from the AAT-VEN and ATZ-VEN (described above) using NEBuilder system (NEB E5520S), with the only the difference between AAT and ATZ is at the codon “GAG”, which is switched to “AAG” at nucleotide number 1043 underlined in the below ORF. .. SS (lactase)-S-FLAG-AAT or SS (lactase)-S-FLAG-ATZ: “S-FLAG” refers to the SNAP-FLAG, which was derived from SNAP-FLAGCD59 (obtained from Addgene, plasmid # 50374, deposited by Reika Watanabe) by PCR amplification.

    Clone Assay:

    Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes
    Article Snippet: For assembly we used NEBuilder (NEB E5520S). .. SS(lactase)-VEN-AAT and SS(lactase)-VEN-ATZ: These were generated by starting with SS(lactase)-CER-TMED9 (described above) and replacing CER with VEN through BsrGI and AgeI restriction cut sites, and then replacing the TMED9 mature domain with AAT and ATZ mature domains cloned from the AAT-VEN and ATZ-VEN (described above) using NEBuilder system (NEB E5520S), with the only the difference between AAT and ATZ is at the codon “GAG”, which is switched to “AAG” at nucleotide number 1043 underlined in the below ORF. .. SS (lactase)-S-FLAG-AAT or SS (lactase)-S-FLAG-ATZ: “S-FLAG” refers to the SNAP-FLAG, which was derived from SNAP-FLAGCD59 (obtained from Addgene, plasmid # 50374, deposited by Reika Watanabe) by PCR amplification.



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    agei restriction cut sites  (New England Biolabs)
    97
    New England Biolabs agei restriction cut sites
    Agei Restriction Cut Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agei+restriction+cut+sites/AgeI/10__1091_slash_mbc__e23___06___0257-250-20-46
    Average 97 stars, based on 1 article reviews
    agei restriction cut sites - by Bioz Stars, 2026-09
    97/100 stars
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